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Persulfur anions of persulfurated cysteine residues and positively charged carbonyl carbons of pyrimidone moiety of inhibitor have a substantial long-range electrostatic interaction, according to theoretical calculations

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These results directly confirm that excessive NNMT activation suppresses NAD+ synthesis and impairs energy metabolism, while NNMT inhibition effectively reverses this process, establishing NNMT as a negative regulator of energy metabolism